A reverse dot-blot method for rapid detection of non-deletion alpha thalassaemia

Br J Haematol. 1999 Mar;104(3):513-5. doi: 10.1046/j.1365-2141.1999.01221.x.


A reverse dot blot method based on membrane-bound allele-specific oligonucleotides as hybridization targets for amplified alpha-gene fragments has been developed for the rapid detection of four non-deletion alpha thalassaemia defects found in the Chinese. Since these non-deletion defects account for 22 8% of haemoglobin H disease, a sensitive, specific and rapid screening method should be of value.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Nucleic Acid Hybridization / methods*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • alpha-Thalassemia / diagnosis*