Potassium Uptake Through the TOK1 K+ Channel in the Budding Yeast

J Membr Biol. 1999 Mar 15;168(2):149-57. doi: 10.1007/s002329900505.

Abstract

The current through TOK1 (YKC1), the outward-rectifying K+ channel in Saccharomyces cerevisiae, was amplified by expressing TOK1 from a plasmid driven by a strong constitutive promoter. TOK1 so hyper-expressed could overcome the K+ auxotrophy of a mutant missing the two K+ transporters, TRK1 and TRK2. This trk1Delta trk2Delta double mutant hyperexpressing the TOK1 transgene had a higher internal K+ content than one expressing the empty plasmid. We examined protoplasts of these TOK1-hyperexpressing cells under a patch clamp. Besides the expected K+ outward current activating at membrane potential (Vm) above the K+ equilibrium potential (EK+), a small inward current was consistently observed when the Vm was slightly below EK+. The inward and the outward currents are similar in their activation rates, deactivation rates, ion specificities and Ba2+ inhibition, indicating that they flow through the same channel. Thus, the yeast outwardly rectifying K+ channel can take up K+ into yeast cells, at least under certain conditions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Potassium / metabolism*
  • Potassium Channels / genetics
  • Potassium Channels / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*

Substances

  • Fungal Proteins
  • Potassium Channels
  • Saccharomyces cerevisiae Proteins
  • TOK1 protein, S cerevisiae
  • Potassium