Crystallization and preliminary x-ray diffraction studies of a novel bacterial esterase

Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):915-7. doi: 10.1107/s0907444998018459.


A novel bacterial esterase has been crystallized in two forms suitable for X-ray diffraction studies. Crystals have been obtained by vapour-phase diffusion at 290 K using ammonium sulfate as precipitant. The first crystals grew in space group C2 with unit-cell parameters a = 134.7, b = 55.8, c = 110.3 A, beta = 125.1 degrees. A monoclinic data set has been collected to 2.0 A resolution. Microseeding yielded a second crystal form which grew in space group P212121 with unit-cell parameters a = 57.1, b = 115.4, c = 130.4 A. Native data from these crystals have been collected to 1.6 A resolution. A molecular envelope has been determined using an uranyl acetate derivative for phase calculation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Crystallization
  • Esterases / chemistry*
  • Rhizobium / enzymology
  • Solvents
  • X-Ray Diffraction


  • Bacterial Proteins
  • Solvents
  • Esterases