Inhibitory effect of erythromycin on interleukin-8 secretion from exudative cells in the nasal discharge of patients with chronic sinusitis

Laryngoscope. 1999 Mar;109(3):407-10. doi: 10.1097/00005537-199903000-00012.

Abstract

Objectives: The mechanism of the efficacy of long-term low-dose macrolide therapy for chronic sinusitis is not fully understood. The authors studied the inhibitory effect of erythromycin on interleukin-8 (IL-8) secretion from exudative cells in the nasal discharge of patients with chronic sinusitis.

Study design and methods: Exudative cells in the nasal discharge were isolated from six patients with nonallergic chronic sinusitis. The cells, more than 90% of which were neutrophils, were incubated with or without erythromycin in the presence of 10 micrograms/mL of lipopolysaccharide. The IL-8 concentrations in the culture supernatants were measured by enzyme-linked immunoassay.

Results: The amount of secreted IL-8 in the absence of erythromycin was 682 +/- 226 pg/10(6) cells/24 h. The IL-8 secretion was significantly reduced to 66 +/- 15% and 46 +/- 13% of the control in the presence of 10(-6) and 10(-5) M of erythromycin, respectively.

Conclusion: Erythromycin may act as a biologic modulator that inhibits IL-8 secretion from exudative cells and thereby blocks the vicious circle of neutrophil recruitment and IL-8 generation in the inflammatory site in chronic sinusitis.

MeSH terms

  • Anti-Bacterial Agents / administration & dosage*
  • Anti-Bacterial Agents / adverse effects
  • Chronic Disease
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Erythromycin / administration & dosage*
  • Erythromycin / adverse effects
  • Humans
  • In Vitro Techniques
  • Interleukin-8 / antagonists & inhibitors*
  • Interleukin-8 / metabolism
  • Long-Term Care
  • Nasal Mucosa / drug effects*
  • Nasal Mucosa / immunology
  • Nasal Mucosa / metabolism
  • Neutrophil Activation / drug effects
  • Neutrophils / drug effects*
  • Neutrophils / immunology
  • Sinusitis / drug therapy*
  • Sinusitis / immunology

Substances

  • Anti-Bacterial Agents
  • Interleukin-8
  • Erythromycin