Six3 overexpression initiates the formation of ectopic retina

Genes Dev. 1999 Mar 15;13(6):649-54. doi: 10.1101/gad.13.6.649.


The homeobox gene sine oculis (so) is essential for visual system formation in Drosophila. A vertebrate member of the so/Six gene family, Six3, is expressed in the developing eye and forebrain. Injection of Six3 RNA into medaka fish embryos causes ectopic Pax6 and Rx2 expression in midbrain and cerebellum, resulting in the formation of ectopic retinal primordia. Injected mouse Six3 RNA initiates ectopic expression of endogenous medaka Six3, uncovering a feedback control of Six3 expression. Initiation of ectopic retina formation reveals a pivotal role for Six3 in vertebrate retina development and hints at a conserved regulatory network underlying vertebrate and invertebrate eye development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers
  • Embryo, Nonmammalian / metabolism
  • Eye Proteins
  • Gene Expression Regulation, Developmental*
  • Homeodomain Proteins / genetics*
  • Mice
  • Nerve Tissue Proteins / genetics*
  • Oryzias / embryology
  • RNA / administration & dosage
  • Retina / embryology
  • Retina / metabolism*
  • Transcription, Genetic


  • DNA Primers
  • Eye Proteins
  • Homeodomain Proteins
  • Nerve Tissue Proteins
  • Sine oculis homeobox homolog 3 protein
  • RNA

Associated data

  • GENBANK/AJ007939
  • GENBANK/AJ132403