Cloning of murine NKG2A, B and C: second family of C-type lectin receptors on murine NK cells

Eur J Immunol. 1999 Mar;29(3):755-61. doi: 10.1002/(SICI)1521-4141(199903)29:03<755::AID-IMMU755>3.0.CO;2-X.


Multiple NK cell receptors for MHC class I have been identified. They include killer inhibitory receptors and CD94/NKG2 heterodimers in humans and the Ly49 family in mice. Here we report the cloning of murine NKG2A, B and C. The deduced amino acid sequence of mouse NKG2A contains only one consensus cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). NKG2A from B6 and BALB/c mice differ by six amino acid residues in the extracellular domain. Murine NKG2B, like its human conterpart, appears to be a splice variant of NKG2A and lacks a large portion of the stalk region. Murine NKG2C lacks an ITIM in its cytoplasmic domain, a feature shared by human and rat NKG2C. However, unlike the human counterpart, the transmembrane domain of mouse NKG2C does not contain a charged amino acid residue. Mouse NKG2A mRNA was detected in IL-2-activated NK cells and spleen cells but not in other tissues. The NKG2A gene was localized on the distal portion of chromosome 6 where the NK complex has been located. These results further extend the repertoire of C-type lectin receptors on murine NK cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD / genetics*
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression
  • Humans
  • Killer Cells, Natural / metabolism*
  • Lectins / metabolism*
  • Lectins, C-Type*
  • Membrane Glycoproteins / genetics*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • NK Cell Lectin-Like Receptor Subfamily C
  • NK Cell Lectin-Like Receptor Subfamily D
  • Rats
  • Receptors, Immunologic / genetics*
  • Receptors, Mitogen / genetics*
  • Receptors, Natural Killer Cell
  • Sequence Analysis, DNA


  • Antigens, CD
  • DNA, Complementary
  • KLRC1 protein, human
  • KLRC2 protein, human
  • KLRD1 protein, human
  • Klrc1 protein, mouse
  • Klrc2 protein, mouse
  • Klrd1 protein, mouse
  • Klrd1 protein, rat
  • Lectins
  • Lectins, C-Type
  • Membrane Glycoproteins
  • NK Cell Lectin-Like Receptor Subfamily C
  • NK Cell Lectin-Like Receptor Subfamily D
  • Receptors, Immunologic
  • Receptors, Mitogen
  • Receptors, Natural Killer Cell

Associated data

  • GENBANK/AF109782
  • GENBANK/AF109783
  • GENBANK/AF109784
  • GENBANK/AF109785