Abstract
A novel tricyclic diterpenoid antibiotic, brasilicardin A, was isolated from the culture broth of Nocardia brasiliensis IFM 0406. The antibiotic exhibited immunosuppressive activity in a mouse mixed lymphocyte reaction (MLR) assay system and its IC50 value was 0.057 microg/ml. Although the inhibitory activity of cyclosporin A (CyA) against IL-2 production was confirmed in the MLR assay system, brasilicardin A did not have the activity. The results of in vitro toxicity testing of brasilicardin A against various human cell lines were compared with those of CyA.
MeSH terms
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Aminoglycosides*
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Animals
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Anti-Bacterial Agents / biosynthesis
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / pharmacology*
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Bacteria / drug effects
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Cell Survival / drug effects
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Cells, Cultured
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Chromatography, High Pressure Liquid
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Fermentation
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Fungi / drug effects
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Humans
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Immunophilins / antagonists & inhibitors
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Immunosuppressive Agents / isolation & purification
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Immunosuppressive Agents / pharmacology*
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Interleukin-2 / metabolism
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Lymphocyte Culture Test, Mixed
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Mice
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Microbial Sensitivity Tests
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Nocardia / metabolism*
Substances
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Aminoglycosides
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Anti-Bacterial Agents
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Immunosuppressive Agents
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Interleukin-2
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brasilicardin A
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Immunophilins