Escherichia coli skp chaperone coexpression improves solubility and phage display of single-chain antibody fragments

Protein Expr Purif. 1999 Apr;15(3):336-43. doi: 10.1006/prep.1999.1035.

Abstract

Expression of single-chain antibody fragments (scAb)in the periplasm of Escherichia coli often results in low soluble product yield and cell lysis. We have increased scAb solubility and prevented cell culture lysis by coexpressing the E. coli Skp chaperone gene. A mutant Skp cistron was linked to a bacteriophage T7 gene 10 translational initiation region and placed either downstream of a scAb gene within an isopropyl beta-d-thiogalactopyranoside-inducible expression cassette or on a separate colE1-compatible arabinose-inducible vector. Increases in scAb solubility reflected the amount of coexpressed Skp. A bacteriophage display vector that was also engineered to coexpress Skp permitted display of a virtually undisplayable scAb and should prove useful in expanding library sizes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Bacteriophages
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Escherichia coli / physiology*
  • Escherichia coli Proteins*
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial*
  • Genetic Vectors
  • Immunoglobulin Fragments / biosynthesis*
  • Molecular Chaperones / genetics*
  • Molecular Chaperones / metabolism*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Recombinant Proteins / biosynthesis
  • Solubility

Substances

  • Bacterial Proteins
  • DNA Primers
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Immunoglobulin Fragments
  • Molecular Chaperones
  • Recombinant Proteins
  • Skp protein, E coli