Paradoxical Decrease of an Adipose-Specific Protein, Adiponectin, in Obesity

Biochem Biophys Res Commun. 1999 Apr 2;257(1):79-83. doi: 10.1006/bbrc.1999.0255.

Abstract

We isolated the human adipose-specific and most abundant gene transcript, apM1 (Maeda, K., et al., Biochem. Biophys. Res. Commun. 221, 286-289, 1996). The apM1 gene product was a kind of soluble matrix protein, which we named adiponectin. To quantitate the plasma adiponectin concentration, we have produced monoclonal and polyclonal antibodies for human adiponectin and developed an enzyme-linked immunosorbent assay (ELISA) system. Adiponectin was abundantly present in the plasma of healthy volunteers in the range from 1.9 to 17.0 mg/ml. Plasma concentrations of adiponectin in obese subjects were significantly lower than those in non-obese subjects, although adiponectin is secreted only from adipose tissue. The ELISA system developed in this study will be useful for elucidating the physiological and pathophysiological role of adiponectin in humans.

MeSH terms

  • Adiponectin
  • Adipose Tissue / metabolism
  • Adult
  • Animals
  • Antibodies / immunology
  • Blotting, Western
  • Body Mass Index
  • Enzyme-Linked Immunosorbent Assay / methods
  • Escherichia coli / genetics
  • Female
  • Hot Temperature
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Linear Models
  • Male
  • Mice
  • Middle Aged
  • Molecular Weight
  • Obesity / blood*
  • Protein Binding
  • Protein Sorting Signals / genetics
  • Proteins / genetics
  • Proteins / immunology
  • Proteins / metabolism*
  • Rabbits
  • Recombinant Proteins / immunology
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism

Substances

  • Adiponectin
  • Antibodies
  • Intercellular Signaling Peptides and Proteins
  • Protein Sorting Signals
  • Proteins
  • Recombinant Proteins