Abstract
NEDD8 is a novel ubiquitin-like protein that has been shown to conjugate to nuclear proteins in a manner analogous to ubiquitination and sentrinization. Recently, human cullin-4A was reported to be conjugated by a single molecule of NEDD8. Here, we show that human cullin-2 is also conjugated by a single molecule of the NEDD8. The C-terminal 171-amino-acid residues in human cullin-2 are sufficient for NEDD8-conjugation. In addition, the equivalent C-terminal fragments of other cullins have been shown to be conjugated by NEDD8. Mapping of the NEDD8-conjugation site revealed that Lys-689 in human cullin-2 is conjugated by NEDD8. Interestingly, the Lys residue at position 689 in cullin-2 is conserved in all cullin family members, including human cullin-1, -2, -3, -4A, -4B, and -5 and yeast cullin (Cdc53), suggesting the possibility that other cullin family members are conjugated by NEDD8/Rub1 at a Lys residue of equivalent position.
Copyright 1999 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Amino Acid Substitution
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Animals
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Binding Sites
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Blotting, Western
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COS Cells
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Cycle Proteins / chemistry
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Conserved Sequence
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Cullin Proteins*
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Humans
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Lysine / genetics
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Lysine / metabolism
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Molecular Sequence Data
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Molecular Weight
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NEDD8 Protein
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Neoplasm Proteins / chemistry
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Neoplasm Proteins / genetics
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Peptide Fragments / chemistry
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Peptide Fragments / genetics
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Peptide Fragments / isolation & purification
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Peptide Fragments / metabolism
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Protein Binding
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Deletion
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Transfection
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Ubiquitins / metabolism*
Substances
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CUL2 protein, human
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CUL4A protein, human
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Carrier Proteins
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Cdc53 protein, S cerevisiae
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Cell Cycle Proteins
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Cullin Proteins
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NEDD8 Protein
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NEDD8 protein, human
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Neoplasm Proteins
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Peptide Fragments
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Ubiquitins
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Lysine