Ballistic transformation of Caenorhabditis elegans

Gene. 1999 Mar 18;229(1-2):31-5. doi: 10.1016/s0378-1119(99)00043-8.

Abstract

A novel method to transform the nematode Caenorhabditis elegans is described. DNA coprecipitated with gold particles is shot at worms by means of a helium beam. Transformed worms are either identified by a dominant visible marker or selected by a conditional lethal system.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Biolistics*
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans Proteins*
  • Collagen / genetics
  • Gold
  • Helium
  • Helminth Proteins / genetics
  • Microscopy, Phase-Contrast
  • Mutation / genetics
  • Particle Size
  • Transformation, Genetic*

Substances

  • Caenorhabditis elegans Proteins
  • Helminth Proteins
  • rol-6 protein, C elegans
  • Helium
  • Gold
  • Collagen