Characterization of protein profiles and cross-reactivity of Blastocystis antigens by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis

Parasitol Res. 1999 Apr;85(4):343-6. doi: 10.1007/s004360050559.


The protein profiles of Blastocystis hominis, B. lapemi, and B. ratti were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and species could be differentiated by this means as well as by Western-blot analysis with polyclonal antibodies. No intraspecies difference could be distinguished between the two B. hominis isolates or the three B. ratti isolates. Western-blot analysis showed extensive cross-reactivity of B. lapemi and B. hominis antigens with anti-B. ratti serum. Some of the cross-reactive antigens were glycoproteins as determined on the basis of their sensitivity to periodate treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Protozoan / immunology*
  • Blastocystis / chemistry*
  • Blastocystis / classification
  • Blastocystis / growth & development
  • Blastocystis / immunology
  • Blastocystis Infections / parasitology*
  • Blotting, Western
  • Cross Reactions
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Protozoan Proteins / analysis*
  • Rabbits
  • Sodium Dodecyl Sulfate


  • Antigens, Protozoan
  • Protozoan Proteins
  • Sodium Dodecyl Sulfate