A novel simple satellite DNA is colocalized with the Stalker retrotransposon in Drosophila melanogaster heterochromatin

Mol Gen Genet. 1999 Mar;261(2):381-7. doi: 10.1007/s004380050979.

Abstract

In the T(1:2)dor(var7) multibreak rearrangement the distal 1A-2B segment of the X chromosome of Drosophila melanogaster is juxtaposed to an inverted portion of the heterochromatin of chromosome 2. Analysis of mitotic chromosomes by a series of banding techniques has permitted us precisely to locate the heterochromatic breakpoint of this translocation in the h42 region of 2R. Cloning and sequencing of the eu-heterochromatic junction revealed that the translocated 1A-2B fragment is joined to (AACAC)n repeats, which represent a previously undescribed satellite DNA in D. melanogaster. These repeated sequences have been estimated to account for about 1 Mb of the D. melanogaster genome. The repeats are located mainly in the Y chromosome and in the heterochromatin of the right arm of chromosome 2 (2Rh), where they are colocalized with the Stalker retrotransposon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping
  • DNA, Complementary
  • DNA, Satellite*
  • Drosophila melanogaster / genetics*
  • Gene Rearrangement
  • Genes, Insect*
  • Heterochromatin*
  • Mitosis
  • Molecular Sequence Data
  • Retroelements*
  • Sequence Analysis, DNA

Substances

  • DNA, Complementary
  • DNA, Satellite
  • Heterochromatin
  • Retroelements

Associated data

  • GENBANK/X86002
  • GENBANK/X86075