Visualization of neuronal form and function in brain slices by infrared videomicroscopy

Abstract

As a standard preparation for neurophysiological experiments, brain slices were introduced some 20 years ago. Although this technique has greatly advanced our understanding of brain physiology, the utility of this preparation has been limited to some extent by the difficulty of visualizing individual neurons in standard thick slices. The use of infrared videomicroscopy has solved this problem. It is now possible to visualize neurons in slices in great detail, and neuronal processes can be patch-clamped under visual control. Infrared videomicroscopy has also been applied successfully to other fields of neuroscience, such as neuronal development and neurotoxicity. A further development of infrared videomicroscopy allows the visualization of the spread of excitation in slices, making the technique a tool for investigating neuronal function and the pharmacology of synaptic transmission.