Proteomics: quantitative and physical mapping of cellular proteins

Trends Biotechnol. 1999 Mar;17(3):121-7. doi: 10.1016/s0167-7799(98)01245-1.


Genome sequencing provides a wealth of information on predicted gene products (mostly proteins), but the majority of these have no known function. Two-dimensional gel electrophoresis and mass spectrometry have, coupled with searches in protein and EST databases, transformed the protein-identification process. The proteome is the expressed protein complement of a genome and proteomics is functional genomics at the protein level. Proteomics can be divided into expression proteomics, the study of global changes in protein expression, and cell-map proteomics, the systematic study of protein-protein interactions through the isolation of protein complexes.

Publication types

  • Review

MeSH terms

  • Biotechnology / trends*
  • Genome
  • Mass Spectrometry / trends*
  • Peptide Mapping / trends*
  • Proteins / analysis
  • Proteins / genetics
  • Proteins / isolation & purification


  • Proteins