The Drosophila melanogaster 60A chromosomal division is extremely dense with functional genes: their sequences, genomic organization, and expression

Genomics. 1999 Apr 1;57(1):43-56. doi: 10.1006/geno.1999.5746.


We cloned and sequenced genomic DNA contigs spanning over 45 kb, surrounding the insertion site of the P-element that is responsible for the developmental defects in the ken and barbie (ken) mutant of Drosophila. This region harbors 10 functional transcription units, in addition to the already well-characterized TGFbeta-60A gene. These include the genes, undefined 1 (UD1), UD2, and UD3, each coding for proteins of unknown function, the ken gene encoding a new Krüppel-like putative transcription factor, the fly homologues of the mammalian mitochondrial trifunctional enzyme (thiolase), and the TAR DNA-binding protein-43 (TBPH), the first nonvertebrate member of the transmembrane 4 superfamily (TM4SF) gene, a new homeodomain gene, and a gene coding for a putative nuclear binding protein (PNBP) that is homologous to maleless, and a Copia-like element. UD3 exists in an intron of the maleless homologue, yet is expressed independent of it. The UD1 and TM4SF genes orient in a tail-to-tail manner with their 3' untranslated region sequences overlapping over 44 nucleotides. Thus the partial overlap and intraintronic organization permitted dense packing of the functional genes within a short segment of the genome.

Publication types

  • Comparative Study

MeSH terms

  • Acetyl-CoA C-Acetyltransferase / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • DNA-Binding Proteins / genetics
  • Databases, Factual
  • Drosophila Proteins / genetics
  • Drosophila melanogaster / genetics*
  • Expressed Sequence Tags
  • Genes, Insect*
  • Homeodomain Proteins / genetics
  • In Situ Hybridization
  • Models, Genetic
  • Molecular Sequence Data
  • Multigene Family
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transforming Growth Factor beta / genetics


  • DNA-Binding Proteins
  • Drosophila Proteins
  • Homeodomain Proteins
  • TBPH protein, Drosophila
  • Transforming Growth Factor beta
  • Acetyl-CoA C-Acetyltransferase

Associated data

  • GENBANK/AB010260
  • GENBANK/AB010261
  • GENBANK/AB010262
  • GENBANK/AB010263
  • GENBANK/AB010264
  • GENBANK/AB010265
  • GENBANK/AB019705
  • GENBANK/AB019706
  • GENBANK/AB019707