High cell density culture of metabolically engineered Escherichia coli for the production of poly(3-hydroxybutyrate) in a defined medium

Biotechnol Bioeng. 1998 Apr 20-May 5;58(2-3):325-8.

Abstract

A recombinant Escherichia coli strain XL1-Blue harboring a stable high-copy-number plasmid pSYL107 containing the Alcaligenes eutrophus polyhydroxyalkanoate biosynthesis genes and the Escherichia coli ftsZ gene was employed for the production of poly(3-hydroxybutyrate) (PHB) by fed-batch culture in a defined medium. Suppression of filamentation by overexpressing the cell division protein FtsZ allowed production of PHB to a high concentration (77 g/L) with high productivity (2 g/L/h) in a defined medium, which was not possible with the recombinant E. coli that underwent filamentation. Further optimization of fed-batch culture condition resulted in PHB concentration of 104 g/L in a defined medium, which was the highest value reported to date by employing recombinant E. coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcaligenes / genetics
  • Alcaligenes / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Cell Culture Techniques
  • Culture Media
  • Cytoskeletal Proteins*
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Genetic Engineering*
  • Hydroxybutyrates / metabolism*
  • Polyesters / metabolism*
  • Polymers

Substances

  • Bacterial Proteins
  • Culture Media
  • Cytoskeletal Proteins
  • FtsZ protein, Bacteria
  • Hydroxybutyrates
  • Polyesters
  • Polymers
  • poly-beta-hydroxybutyrate