Selection analysis of single-stranded clones using solid-phase techniques

Genet Anal. 1999 Apr;15(2):47-50. doi: 10.1016/s1050-3862(98)00035-7.


An efficient selection analysis of single-stranded DNA fragments using subtractive hybridization on magnetic beads and fluorescence-based fragment analysis is described. This approach facilitates either direct cloning of captured fragments or removal of common clones from cDNA or shot-gun clone libraries.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA, Single-Stranded / analysis*
  • Humans
  • Mice
  • Nucleic Acid Hybridization / methods*


  • DNA, Single-Stranded