Detection of Chlamydia pneumoniae DNA in atheromatous tissues by polymerase chain reaction

Wien Klin Wochenschr. 1999 Feb 26;111(4):153-6.


Chlamydia pneumoniae is an important human respiratory pathogen. Recently, seroepidemiological data and the demonstration of chlamydial DNA or antigen within parts of atherosclerotic lesions have suggested a causal association between chlamydial infections and atherosclerosis. As the results obtained by different groups show considerable variations, we provide further data based on a highly specific and sensitive nested PCR method. Positivity was confirmed by nonradioactive hybridization with a specific probe, and sensitivity was determined by titration experiments. C. pneumoniae DNA was detected in 8/29 (28%) of carotid artery samples and 3/14 (21%) of aortic aneurysms.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Arteriosclerosis / pathology*
  • Arteriosclerosis / surgery
  • Carotid Arteries / pathology
  • Carotid Stenosis / pathology*
  • Carotid Stenosis / surgery
  • Chlamydia Infections / pathology*
  • Chlamydophila pneumoniae*
  • DNA, Bacterial / analysis*
  • Endarterectomy, Carotid
  • Female
  • Humans
  • Male
  • Middle Aged
  • Polymerase Chain Reaction*
  • Sensitivity and Specificity


  • DNA, Bacterial