Prostanoid receptors involved in the relaxation of human bronchial preparations

Br J Pharmacol. 1999 Feb;126(4):867-72. doi: 10.1038/sj.bjp.0702392.


1. Iloprost and cicaprost (IP-receptor agonists) induced relaxations in the histamine- (50 microM) contracted human bronchial preparations (pD2 values, 6.63+/-0.12 and 6.86+/-0.08; Emax values, 90+/-04 and 65+/-08% of the papaverine response for iloprost (n=6) and cicaprost (n=3), respectively). 2. Prostaglandin E2 (PGE2) and misoprostol (EP-receptor agonist) relaxed the histamine-contracted human bronchial preparations (pD2 values, 7.13+/-0.07 and 6.33+/-0.28; Emax values, 67+/-04 and 57+/-08% of the papaverine response for PGE2 (n=14) and misoprostol (n=4), respectively). In addition, both relaxations were inhibited by AH6809 (DP/EP1/EP2-receptor antagonist; 3 microM; n=5-6). 3. The PGE2-induced relaxations of human bronchial preparations were not modified by treatment with AH23848B (TP/EP4-receptor antagonist; 30 microM; n=4). 4. The contracted human bronchial preparations were significantly relaxed by prostaglandin D2 (PGD2) or by BW245C a DP-receptor agonist. However, these responses did not exceed 40% of the relaxation induced by papaverine. In addition, the relaxations induced by PGD2 were significantly inhibited by treatment with a DP-receptor antagonist BWA868C (0.1 microM; n=3). 5. These data suggest that the relaxation of human isolated bronchial preparations induced by prostanoids involved IP-, EP2- and to a lesser extent DP-receptors but not EP4-receptor.

MeSH terms

  • Aged
  • Bronchi / physiology*
  • Female
  • Humans
  • Hydantoins / pharmacology
  • Iloprost / pharmacology
  • In Vitro Techniques
  • Male
  • Middle Aged
  • Muscle Relaxation*
  • Prostaglandin D2 / pharmacology
  • Prostaglandins E / pharmacology
  • Receptors, Prostaglandin / physiology*


  • Hydantoins
  • Prostaglandins E
  • Receptors, Prostaglandin
  • BW 245C
  • Iloprost
  • Prostaglandin D2