Caspases: preparation and characterization

Methods. 1999 Apr;17(4):313-9. doi: 10.1006/meth.1999.0745.


Caspases and their involvement in programmed cell death have been an area of significant interest since their initial identification in 1992. To facilitate the search for new components involved in cell death, and to aid researchers in understanding the interactions between currently known cell death proteins, we describe a number of techniques commonly used in the preparation and characterization of caspases.

MeSH terms

  • Apoptosis*
  • Caspases / biosynthesis
  • Caspases / chemistry*
  • Caspases / isolation & purification
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression / genetics
  • Kinetics
  • Protease Inhibitors / pharmacology
  • Spectrophotometry
  • Substrate Specificity


  • Protease Inhibitors
  • Caspases