Cloning and chromosomal localization of human Cdc42-binding protein kinase beta

Genomics. 1999 Apr 15;57(2):297-300. doi: 10.1006/geno.1999.5769.

Abstract

The p21 GTPases, Rho and Cdc42, regulate numerous cellular functions by binding to members of a serine/threonine protein kinase subfamily. These functions include the remodeling of the cell cytoskeleton that is a feature of cell growth and differentiation. Two of these p21 GTPase-regulated kinases, the myotonic dystrophy protein kinase-related Cdc42-binding kinases (MRCKalpha and beta), have been recently characterized in rat. Both of these proteins phosphorylate nonmuscle myosin light chain, a prerequisite for the activation of actin-myosin contractility. Here we report the cDNA cloning of the human homologue of MRCKbeta, CDC42BPB, which was found by Northern blot analysis to be expressed in a wide range of tissues. The human CDC42BPB gene maps to cytogenetic band 14q32.3 by FISH analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Northern
  • Chromosome Mapping
  • Chromosomes, Human, Pair 14 / genetics*
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Myotonin-Protein Kinase
  • Protein-Tyrosine Kinases / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Analysis, DNA
  • Tissue Distribution

Substances

  • DNA, Complementary
  • RNA, Messenger
  • CDC42BPB protein, human
  • Protein-Tyrosine Kinases
  • Myotonin-Protein Kinase

Associated data

  • GENBANK/AF128625