The Chicken HPRT Gene: A Counter Selectable Marker for the DT40 Cell Line

Nucleic Acids Res. 1999 May 1;27(9):1966-9. doi: 10.1093/nar/27.9.1966.

Abstract

We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will enhance the utility of the hyper-recombinogenic DT40 cell line as a system for the genetic analysis of cell autonomous functions in vertebrates and as a tool for mammalian chromosome engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line, Transformed*
  • Chickens
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Primers
  • Genetic Engineering
  • Genetic Markers*
  • Humans
  • Hypoxanthine Phosphoribosyltransferase / genetics*
  • Molecular Sequence Data
  • Recombination, Genetic
  • Sequence Homology, Amino Acid

Substances

  • DNA Primers
  • Genetic Markers
  • Hypoxanthine Phosphoribosyltransferase