Abstract
The identities of the four floral organ types in an Arabidopsis flower are specified by the combinatorial activities of the floral homeotic A, B, and C function genes; AGAMOUS is the only known C function gene. We have identified two genes that interact with AG in the specification of floral structure, HUA1 and HUA2, from a screen for enhancers of a weak ag allele, ag-4. HUA1 and HUA2 are involved in all aspects of AG function. HUA2 encodes a novel protein that contains nuclear localization signals and signature motifs that suggest HUA2, like AG, may be a transcription factor. Molecular analyses suggest that HUA2 (and possibly HUA1) acts to facilitate AG action at the same hierarchical level as AG.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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AGAMOUS Protein, Arabidopsis
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Alleles
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Amino Acid Sequence
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Arabidopsis / cytology
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Arabidopsis / genetics*
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Arabidopsis / growth & development
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Arabidopsis / metabolism
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Arabidopsis Proteins*
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Cell Differentiation
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Chromosome Mapping
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Cloning, Molecular
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Dosage
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Gene Expression Regulation, Plant
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Genes, Homeobox
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Genes, Plant / genetics
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Genetic Complementation Test
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Genotype
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Homeodomain Proteins / genetics*
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Homeodomain Proteins / metabolism
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MADS Domain Proteins
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Molecular Sequence Data
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Mutation
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Phenotype
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Plant Proteins / metabolism*
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Plant Proteins / physiology
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Plant Structures / cytology
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Plant Structures / growth & development*
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Plant Structures / metabolism
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Plant Structures / ultrastructure
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RNA, Messenger / analysis
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RNA, Messenger / metabolism
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / physiology
Substances
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AGAMOUS Protein, Arabidopsis
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AP1 protein, Arabidopsis
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Arabidopsis Proteins
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DNA-Binding Proteins
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HUA2 protein, Arabidopsis
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Homeodomain Proteins
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MADS Domain Proteins
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Plant Proteins
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RNA, Messenger
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Transcription Factors