Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators

Mol Cell. 1999 Mar;3(3):361-70. doi: 10.1016/s1097-2765(00)80463-3.


The human thyroid hormone receptor-associated protein (TRAP) complex, an earlier described coactivator for nuclear receptors, and an SRB- and MED-containing cofactor complex (SMCC) that mediates activation by Gal4-p53 are shown to be virtually the same with respect to specific polypeptide subunits, coactivator functions, and mechanisms of action (activator interactions). In parallel with ligand-dependent interactions of nuclear receptors with the TRAP220 subunit, p53 and VP16 activation domains interact directly with a newly cloned TRAP80 subunit. These results indicate novel pathways for the function of nuclear receptors and other activators (p53 and VP16) through a common coactivator complex that is likely to target RNA polymerase II. Identification of the TRAP230 subunit as a previously predicted gene product also suggests a coactivator-related transcription defect in certain disease states.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Blotting, Northern
  • Blotting, Western
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Cloning, Molecular
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Gene Expression Regulation
  • HeLa Cells
  • Herpes Simplex Virus Protein Vmw65 / metabolism
  • Humans
  • Mediator Complex
  • Mediator Complex Subunit 1
  • Molecular Sequence Data
  • Protein Binding
  • RNA Polymerase II / metabolism
  • Receptors, Calcitriol / metabolism
  • Receptors, Thyroid Hormone / metabolism*
  • Response Elements / genetics
  • Saccharomyces cerevisiae Proteins*
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Tumor Suppressor Protein p53 / metabolism


  • Carrier Proteins
  • Fungal Proteins
  • Herpes Simplex Virus Protein Vmw65
  • MED1 protein, human
  • MED14 protein, human
  • MED6 protein, human
  • Med17 protein, human
  • Mediator Complex
  • Mediator Complex Subunit 1
  • Receptors, Calcitriol
  • Receptors, Thyroid Hormone
  • SRB2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors
  • Tumor Suppressor Protein p53
  • RNA Polymerase II

Associated data

  • GENBANK/AF117657
  • GENBANK/AF117754
  • GENBANK/AF117755
  • GENBANK/AF117756
  • GENBANK/AF121228
  • GENBANK/AF135802