Evectins: vesicular proteins that carry a pleckstrin homology domain and localize to post-Golgi membranes

Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4633-8. doi: 10.1073/pnas.96.8.4633.

Abstract

We have identified two vesicular proteins, designated evectin (evt)-1 and -2. These proteins are approximately 25 kDa in molecular mass, lack a cleaved N-terminal signal sequence, and appear to be inserted into membranes through a C-terminal hydrophobic anchor. They also carry a pleckstrin homology domain at their N termini, which potentially couples them to signal transduction pathways that result in the production of lipid second messengers. evt-1 is specific to the nervous system, where it is expressed in photoreceptors and myelinating glia, polarized cell types in which plasma membrane biosynthesis is prodigious and regulated; in contrast, evt-2 is widely expressed in both neural and nonneural tissues. In photoreceptors, evt-1 localizes to rhodopsin-bearing membranes of the post-Golgi, an important transport compartment for which specific molecular markers have heretofore been lacking. The structure and subcellular distribution of evt-1 strongly implicate this protein as a mediator of post-Golgi trafficking in cells that produce large membrane-rich organelles. Its restricted cellular distribution and genetic locus make it a candidate gene for the inherited human retinopathy autosomal dominant familial exudative vitreoretinopathy and suggest that it also may be a susceptibility gene for multiple sclerosis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Central Nervous System / metabolism*
  • Cloning, Molecular
  • Golgi Apparatus / metabolism*
  • Humans
  • Intracellular Membranes / metabolism
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins / analysis
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Multigene Family
  • Organ Specificity
  • Photoreceptor Cells / metabolism
  • RNA, Messenger / analysis
  • Ranidae
  • Rats
  • Recombinant Proteins / analysis
  • Recombinant Proteins / biosynthesis
  • Retina / metabolism
  • Second Messenger Systems
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Transcription, Genetic
  • src Homology Domains

Substances

  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • PLEKHB1 protein, human
  • PLEKHB2 protein, human
  • Plekhb1 protein, mouse
  • Plekhb1 protein, rat
  • Plekhb2 protein, mouse
  • RNA, Messenger
  • Recombinant Proteins

Associated data

  • GENBANK/AF118562
  • GENBANK/AF189817