Active and passive immunization with the Pseudomonas V antigen protects against type III intoxication and lung injury

Nat Med. 1999 Apr;5(4):392-8. doi: 10.1038/7391.


Pseudomonas aeruginosa is an opportunistic bacterial pathogen that can cause fatal acute lung infections in critically ill individuals. Damage to the lung epithelium is associated with the expression of toxins that are directly injected into eukaryotic cells through a type Ill-mediated secretion and translocation mechanism. Here we show that the P. aeruginosa homolog of the Yersinia V antigen, PcrV, is involved in the translocation of type III toxins. Vaccination against PcrV ensured the survival of challenged mice and decreased lung inflammation and injury. Antibodies to PcrV inhibited the translocation of type III toxins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Bacterial / pharmacology
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / poisoning
  • Antigens, Bacterial / therapeutic use*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Bacterial Proteins / poisoning*
  • Bacterial Toxins / genetics
  • Bacterial Toxins / poisoning
  • Bacterial Toxins / therapeutic use*
  • Biological Transport
  • Cell Survival
  • Genes, Bacterial
  • Immunization / methods*
  • Immunization, Passive / methods
  • Lung Diseases / therapy*
  • Macrophages / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Phagocytosis
  • Pore Forming Cytotoxic Proteins
  • Pseudomonas Infections / therapy*
  • Vaccination / methods


  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • Bacterial Toxins
  • Pore Forming Cytotoxic Proteins
  • antigen V, Pseudomonas