Dendritic Cells Directly Trigger NK Cell Functions: Cross-Talk Relevant in Innate Anti-Tumor Immune Responses in Vivo

Nat Med. 1999 Apr;5(4):405-11. doi: 10.1038/7403.

Abstract

Cytotoxic T lymphocytes and natural killer cells are essential effectors of anti-tumor immune responses in vivo. Dendritic cells (DC) 'prime' tumor antigen-specific cytotoxic T lymphocytes; thus, we investigated whether DC might also trigger the innate, NK cell-mediated anti-tumor immunity. In mice with MHC class I-negative tumors, adoptively transferred- or Flt3 ligand-expanded DC promoted NK cell-dependent anti-tumor effects. In vitro studies demonstrated a cell-to-cell contact between DC and resting NK cells that resulted in a substantial increase in both NK cell cytolytic activity and IFN-gamma production. Thus, DC are involved in the interaction between innate and adaptive immune responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Cell Communication
  • Coculture Techniques
  • Cytotoxicity, Immunologic*
  • DNA-Binding Proteins
  • Dendritic Cells / immunology*
  • Killer Cells, Natural / immunology*
  • Ligands
  • Lymphocyte Activation
  • Major Histocompatibility Complex / immunology
  • Membrane Proteins / immunology
  • Mice
  • Mice, Inbred Strains
  • Mice, Mutant Strains
  • Neoplasms, Experimental / classification
  • Neoplasms, Experimental / immunology*

Substances

  • DNA-Binding Proteins
  • Ligands
  • Membrane Proteins
  • Rag2 protein, mouse
  • V(D)J recombination activating protein 2
  • flt3 ligand protein