Activation of nuclear transcription factor kappaB in rat carrageenin-induced pleurisy

Eur J Pharmacol. 1999 Mar 19;369(2):233-6. doi: 10.1016/s0014-2999(99)00087-4.


In this study we investigated the activation of nuclear factor-kappaB in the carrageenin-induced rat pleurisy. We found that nuclear factor-kappaB DNA binding activity, measured in inflammatory cells which migrated into the pleural cavity, was detectable at 3 and 6 h, markedly increased at 24 h and decreased at 48 h after induction of the inflammation. The increase in nuclear factor-kappaB DNA binding activity paralleled both exudate formation and leukocyte infiltration. Treatment of animals with pyrrolidine dithiocarbamate, an inhibitor of nuclear factor-kappaB activation, inhibited the nuclear factor-kappaB DNA binding activity as well as exudate formation and leukocyte infiltration. These results indicate that nuclear factor-kappaB is activated in the carrageenin-induced pleurisy and suggest that its inhibition may represent a novel strategy for the modulation of inflammatory response.

MeSH terms

  • Animals
  • Carrageenan / toxicity*
  • Cell Movement
  • DNA-Binding Proteins / metabolism*
  • Exudates and Transudates / metabolism
  • Leukocytes / physiology
  • Male
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / metabolism*
  • Pleurisy / chemically induced*
  • Pyrrolidines / pharmacology*
  • Rats
  • Rats, Wistar
  • Thiocarbamates / pharmacology*
  • Time Factors


  • DNA-Binding Proteins
  • NF-kappa B
  • Pyrrolidines
  • Thiocarbamates
  • pyrrolidine dithiocarbamic acid
  • Carrageenan