Pathogenicity of basidiospores of Filobasidiella neoformans var. neoformans

Med Mycol. 1998 Dec;36(6):419-24.


Basidiospores of Filobasidiella neoformans var. neoformans (progeny of Cryptococcus neoformans MT 100.1 x VR 45980) were able to induce cryptococcosis in Swiss albino mice if inoculated by intraperitoneal injection, nasal instillation or nasal spraying. The latter method, with the aid of a jet nebulizer, was first adopted to imitate the natural entrance of infectious particles. Using this method the small number of basidiospores (7 x 10(3)) could induce cryptococcosis in mice, while the higher number of the parental laboratory-grown yeast cells (1.5 x 10(6)) did not produce infections. By nasal instillation Cyclophosphamide (Cy)-treated mice were more susceptible to the basidiospores, showing 80% cryptococcosis (eight of 10). Seven of the eight infected mice had disseminated cryptococcosis. Immunocompetent mice were more resistant to basidiospore infection than Cy-treated mice, as 40% of that group developed only pulmonary cryptococcosis; none had disseminated infection. Thus, we propose that basidiospores are one form of the infectious propagules of F. neoformans var. neoformans which can cause cryptococcosis, particularly in immunocompromised people.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basidiomycota / growth & development
  • Basidiomycota / pathogenicity*
  • Cryptococcosis / microbiology*
  • Cryptococcosis / transmission
  • Cryptococcus neoformans / growth & development
  • Cryptococcus neoformans / pathogenicity*
  • Cyclophosphamide / administration & dosage
  • Immunocompetence
  • Immunocompromised Host
  • Immunosuppressive Agents / administration & dosage
  • Lung Diseases, Fungal / microbiology*
  • Lung Diseases, Fungal / transmission
  • Mice
  • Spores, Fungal / pathogenicity


  • Immunosuppressive Agents
  • Cyclophosphamide