Mycobacterium tuberculosis aerogenic rechallenge infections in B cell-deficient mice

Tuber Lung Dis. 1997;78(5-6):257-61. doi: 10.1016/s0962-8479(97)90006-x.

Abstract

Objective: Use gene disrupted mice to examine the possible role of secretory antibody on resistance to re-exposure to pulmonary tuberculosis.

Design: Mice deficient in B cells due to targeted gene disruption were infected by aerosol exposure with Mycobacterium tuberculosis. A further set were identically exposed then given isoniazid to clear the infection and establish a state of memory immunity.

Results: Control of the aerosol infection and generation of gamma interferon proceeded in a similar manner in both naive and memory immune mice, regardless of B cell deficiency.

Conclusions: The absence of antibody responses did not affect the course of infection, thus confirming the classical literature that antibody plays no significant protective role.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aerosols
  • Animals
  • B-Lymphocytes / immunology*
  • Female
  • Gene Expression
  • Immune Tolerance*
  • Immunologic Memory*
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / genetics
  • Lung / immunology
  • Lung / microbiology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • RNA, Messenger / genetics
  • Tuberculosis, Pulmonary / immunology*
  • Tuberculosis, Pulmonary / microbiology

Substances

  • Aerosols
  • RNA, Messenger
  • Interferon-gamma