Bone marrow and recombinant human bone morphogenetic protein-2 in osseous repair

Clin Orthop Relat Res. 1999 Apr;(361):216-27. doi: 10.1097/00003086-199904000-00028.

Abstract

Bone marrow stem cells and recombinant human bone morphogenetic protein-2 each has the capacity to repair osseous defects. Recombinant human bone morphogenetic proteins require the presence of progenitor cells to function. It is hypothesized that a composite graft of recombinant human bone morphogenetic protein-2 and marrow would be synergistic and could result in superior grafting to autogenous bone graft. Syngeneic Lewis rats with a 5-mm critical sized femoral defect were grafted with recombinant human bone morphogenetic protein-2 and marrow, recombinant human bone morphogenetic protein-2, marrow, syngeneic cancellous bone graft, or carrier alone (control). Serial radiographs (3, 6, 9, 12 weeks) and torque testing (12 weeks) were performed. Bone formation and union were determined. The recombinant human bone morphogenetic protein-2 and marrow composite grafts achieved 100% union at 6 weeks. Recombinant human bone morphogenetic protein alone achieved 80% union by week 12. Both groups yielded a higher union rate and superior mechanical properties than did either syngeneic bone graft (38%) or marrow (47%) alone. The superior performance of recombinant human bone morphogenetic protein-2 combined with bone marrow in comparison with each component alone strongly supports a biologic synergism. This experimentation shows the clinical importance of establishing operative site proximity for the osteoinductive factors and responding progenitor cells.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Biocompatible Materials
  • Bone Diseases / diagnostic imaging
  • Bone Diseases / surgery
  • Bone Marrow Transplantation*
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins / therapeutic use*
  • Bone Transplantation / methods
  • Drug Carriers
  • Femur / diagnostic imaging
  • Femur / surgery*
  • Follow-Up Studies
  • Humans
  • Lactic Acid
  • Male
  • Osteogenesis / drug effects
  • Osteogenesis / physiology
  • Polyglycolic Acid
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polymers
  • Radiography
  • Rats
  • Rats, Inbred Lew
  • Recombinant Proteins
  • Torque
  • Transforming Growth Factor beta / therapeutic use*
  • Transplantation, Autologous
  • Transplantation, Isogeneic
  • Wound Healing / drug effects
  • Wound Healing / physiology

Substances

  • BMP2 protein, human
  • Biocompatible Materials
  • Bmp2 protein, rat
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins
  • Drug Carriers
  • Polymers
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • recombinant human bone morphogenetic protein-2
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid