Crystallization and preliminary crystallographic studies of Sfp: a phosphopantetheinyl transferase of modular peptide synthetases

Acta Crystallogr D Biol Crystallogr. 1999 May;55(Pt 5):1098-100. doi: 10.1107/s0907444999003674.

Abstract

The Bacillus subtilis Sfp protein is required for the non-ribosomal biosynthesis of the lipoheptapeptide antibiotic surfactin. It converts seven peptidyl carrier protein (PCP) domains of the surfactin synthetase SfrA-(A-C) to their active holo-forms by 4'-phosphopantetheinylation. The B. subtilis sfp gene was overexpressed in Escherichia coli and its gene product was purified to homogeneity and crystallized. Well diffracting single crystals were obtained from Sfp as well as from a selenomethionyl derivative, using sodium formate as a precipitant. The crystals belong to the tetragonal space group P41212/P43212, with unit-cell parameters a = b = 65.3, c = 150.5 A. They diffract beyond 2.8 A and contain one molecule in the asymmetric unit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / enzymology
  • Bacterial Proteins / chemistry*
  • Cloning, Molecular
  • Crystallization
  • Crystallography, X-Ray
  • Escherichia coli / genetics
  • Lipopeptides
  • Peptide Synthases / chemistry*
  • Peptides, Cyclic*
  • Transferases (Other Substituted Phosphate Groups) / biosynthesis
  • Transferases (Other Substituted Phosphate Groups) / chemistry*
  • Transferases (Other Substituted Phosphate Groups) / genetics
  • Transferases (Other Substituted Phosphate Groups) / isolation & purification

Substances

  • Bacterial Proteins
  • Lipopeptides
  • Peptides, Cyclic
  • phosphopantetheinyl transferase
  • surfactin peptide
  • Transferases (Other Substituted Phosphate Groups)
  • Peptide Synthases
  • surfactin synthetase