The Mycelium-Associated Streptomyces Reticuli Catalase-Peroxidase, Its Gene and Regulation by FurS

Microbiology. 1999 Mar;145 ( Pt 3):549-559. doi: 10.1099/13500872-145-3-549.

Abstract

During early stages of growth, Streptomyces reticuli synthesizes a hyphae-associated, haem-containing enzyme which exhibits catalase and peroxidase activities with broad substrate specificity (CpeB). The purified dimeric enzyme (160 kDa) consists of two identical subunits. Using anti-CpeB antibodies and an expression- as well as a mini-library, the corresponding cpeB gene was identified and sequenced. It encodes a protein of 740 aa with a molecular mass of 81.3 kDa. The deduced protein shares the highest level of amino acid identity with KatG from Caulobacter crescentus and Mycobacterium tuberculosis, and PerA from Bacillus stearothermophilus. Streptomyces lividans transformants carrying cpeB and the upstream-located furS gene with its regulatory region on the bifunctional vector pWHM3 produced low or enhanced levels of CpeB in the presence or absence of Fe ions, respectively. An in-frame deletion of the major part of furS induces increased CpeB synthesis. The data imply that FurS regulates the transcription of cpeB. The deduced FurS protein is rich in histidine residues, contains a putative N-terminally situated helix-turn-helix motif and has a molecular mass of 15.1 kDa. It shares only 29% amino acid identity with the Escherichia coli ferric uptake regulator (Fur) protein, but about 64% with FurA deduced from the genomic sequences of several mycobacteria. The predicted secondary structures of FurS and FurA are highly similar and considerably divergent from those of the E. coli Fur. In contrast to some Gram-negative bacteria, within several mycobacteria an intact furA gene or a furA pseudogene is upstream of a catalase-peroxidase (katG) gene predicted to encode a functional or a non-functional (Mycobacterium leprae) enzyme. Thus the data obtained for Streptomyces reticuli are expected to serve as an additional model to elucidate the regulation of mycobacterial catalase-peroxidase genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Catalase / biosynthesis
  • Catalase / classification
  • Catalase / genetics*
  • Catalase / isolation & purification
  • Cloning, Molecular
  • Dimerization
  • Evolution, Molecular
  • Gene Expression Regulation, Bacterial*
  • Gene Expression Regulation, Enzymologic
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Recombinant Proteins / biosynthesis
  • Repressor Proteins / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Streptomyces / enzymology
  • Streptomyces / genetics*
  • Transcription Factors / metabolism*

Substances

  • Bacterial Proteins
  • FurS protein, Streptomyces reticuli
  • Recombinant Proteins
  • Repressor Proteins
  • Transcription Factors
  • ferric uptake regulating proteins, bacterial
  • Catalase

Associated data

  • GENBANK/AJ132989