Inhibition of peroxynitrite dependent DNA base modification and tyrosine nitration by the extra virgin olive oil-derived antioxidant hydroxytyrosol

Free Radic Biol Med. 1999 Mar;26(5-6):762-9. doi: 10.1016/s0891-5849(98)00231-7.

Abstract

Hydroxytyrosol is one of the o-diphenolic compounds in extra virgin olive oil and has been suggested to be a potent antioxidant. The superoxide radical (O2*-) and nitric oxide (NO*) can react very rapidly to form peroxynitrite (ONOO ), a reactive tissue damaging species thought to be involved in the pathology of several chronic diseases. Hydroxytyrosol was highly protective against the peroxynitrite-dependent nitration of tyrosine and DNA damage by peroxynitrite in vitro. Given that extra virgin olive oil is consumed daily by many humans, hydroxytyrosol derived from this diet could conceivably provide a defense against damage by oxidants in vivo. The biological activity of hydroxytyrosol in vivo will depend on its intake, uptake and access to cellular compartments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Cattle
  • Chronic Disease
  • DNA / chemistry
  • DNA / drug effects*
  • DNA Damage*
  • Humans
  • Hybrid Cells
  • Mice
  • Neuroblastoma
  • Neurons
  • Nitrates / chemistry*
  • Nitrates / pharmacology*
  • Nitric Oxide / chemistry
  • Olive Oil
  • Oxidants / chemistry
  • Oxidants / pharmacology
  • Phenylethyl Alcohol / analogs & derivatives*
  • Phenylethyl Alcohol / chemistry
  • Phenylethyl Alcohol / pharmacology
  • Plant Oils*
  • Rats
  • Retina
  • Superoxides / chemistry
  • Tyrosine / chemistry

Substances

  • Antioxidants
  • Nitrates
  • Olive Oil
  • Oxidants
  • Plant Oils
  • 3,4-dihydroxyphenylethanol
  • Superoxides
  • peroxynitric acid
  • Nitric Oxide
  • Tyrosine
  • DNA
  • Phenylethyl Alcohol