Seroprevalence and disease association of antineutrophil cytoplasmic autoantibodies and antigens in HIV infection

Infection. Mar-Apr 1999;27(2):92-6. doi: 10.1007/BF02560504.

Abstract

This prospective study was designed to determine the role of antineutrophil cytoplasmic autoantibodies (ANCA) in HIV-infected patients. Immunofluorescence tests (IFT) and enzyme-linked immunosorbent assays (ELISA) were applied to sera of 199 consecutive outpatients. In the IFT 20% were positive. An atypical ANCA pattern was demonstrated in 67% of these, 33% revealed a perinuclear staining (pANCA). Specific ELISA revealed proteinase 3 (n = 2), myeloperoxidase (n = 1), lysozyme (n = 2), lactoferrin (n = 1), cathepsin G (n = 1), and human leukocyte elastase (HLE, n = 6). The target antigen remained unidentified in 26 patients. Perinuclear ANCA-positive patients showed atypical antigens in eight of 13 cases; all six patients with anti-HLE revealed a pANCA pattern. The antigens of atypical ANCA-positive patients remained unidentified in 21 of 26 (81%) cases. No signs of vasculitis were present in the ANCA-positive patients. ANCA are frequently found in the sera of HIV-positive patients. They bind to a variety of antigens. No correlation was found between ANCA positivity and autoimmune or opportunistic diseases.

MeSH terms

  • AIDS-Related Opportunistic Infections / diagnosis
  • Adult
  • Antibodies, Antineutrophil Cytoplasmic / blood*
  • Antibodies, Antineutrophil Cytoplasmic / isolation & purification
  • Antigens, Viral / blood*
  • Antigens, Viral / isolation & purification
  • Cohort Studies
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / blood
  • Female
  • Fluorescent Antibody Technique, Indirect
  • HIV Infections / blood
  • HIV Infections / epidemiology
  • HIV Infections / immunology*
  • Humans
  • Male
  • Prospective Studies
  • Seroepidemiologic Studies

Substances

  • Antibodies, Antineutrophil Cytoplasmic
  • Antigens, Viral
  • Epitopes