Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases

Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5322-7. doi: 10.1073/pnas.96.9.5322.

Abstract

Mutation of the PRL1 gene, encoding a regulatory WD protein, results in glucose hypersensitivity and derepression of glucose-regulated genes in Arabidopsis. The yeast SNF1 protein kinase, a key regulator of glucose signaling, and Arabidopsis SNF1 homologs AKIN10 and AKIN11, which can complement the Deltasnf1 mutation, were found to interact with an N-terminal domain of the PRL1 protein in the two-hybrid system and in vitro. AKIN10 and AKIN11 suppress the yeast Deltasnf4 mutation and interact with the SNF4p-activating subunit of SNF1. PRL1 and SNF4 bind independently to adjacent C-terminal domains of AKIN10 and AKIN11, and these protein interactions are negatively regulated by glucose in yeast. AKIN10 and AKIN11, purified in fusion with glutathione S-transferase, undergo autophosphorylation and phosphorylate a peptide of sucrose phosphate synthase in vitro. The sucrose phosphate synthase-peptide kinase activity of AKIN complexes detected by immunoprecipitation is stimulated by sucrose in light-grown Arabidopsis plants. In comparison with wild type, the activation level of AKIN immunocomplexes is higher in the prl1 mutant, suggesting that PRL1 is a negative regulator of Arabidopsis SNF1 homologs. This conclusion is supported by the observation that PRL1 is an inhibitor of AKIN10 and AKIN11 in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins*
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Gene Expression Regulation, Plant*
  • Genes, Fungal
  • Genes, Plant
  • Intracellular Signaling Peptides and Proteins*
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Protein Binding
  • Protein Serine-Threonine Kinases / genetics*
  • Protein Serine-Threonine Kinases / metabolism
  • Sequence Alignment

Substances

  • Arabidopsis Proteins
  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • PRL1 protein, Arabidopsis
  • PRL1 protein, plant
  • Plant Proteins
  • SNF1-related protein kinases
  • Protein Serine-Threonine Kinases

Associated data

  • GENBANK/AJ006021
  • GENBANK/X99279