Abstract
Beta-glucuronidase cleavage of 4-methylumbelliferyl beta-D-glucuronide generates the highly fluorescent compound, 4-methylumbelliferone. We show that other beta-D-glucuronide compounds act as competitors in this assay. The 4-methylumbelliferyl beta-D-glucuronide cleavage assay can easily be adapted to high throughput formats to detect the presence of beta-D glucuronides generated using recombinant glycosyl transferase preparations.
MeSH terms
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Binding, Competitive
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Fluorescence
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Glucuronates / analysis*
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Glucuronates / metabolism*
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Glucuronidase / genetics
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Glucuronidase / metabolism
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Glucuronosyltransferase / genetics
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Glucuronosyltransferase / metabolism*
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Hymecromone / analogs & derivatives
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Hymecromone / metabolism
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Kinetics
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Recombinant Proteins / metabolism*
Substances
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Glucuronates
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Recombinant Proteins
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Hymecromone
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4-methylumbelliferyl glucuronide
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UDP-glucuronosyltransferase, UGT1A6
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Glucuronosyltransferase
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Glucuronidase