A mitogen-activated protein kinase (MPKA) is involved in polarized growth in the filamentous fungus, Aspergillus nidulans

FEMS Microbiol Lett. 1999 Apr 1;173(1):117-25. doi: 10.1111/j.1574-6968.1999.tb13492.x.

Abstract

An Aspergillus nidulans kinase gene, which encodes a protein kinase with high similarity to mitogen-activated protein kinases involved in cell wall construction and morphogenesis in yeast species, was cloned and sequenced. Targeted deletion of the Aspergillus nidulans kinase gene indicates that this kinase is involved in germination of conidial spores and polarized growth. These defects were largely remedied on complex high osmolarity media, although abnormal swellings of hyphal tips were still observed. Glycerol (1 M) only supported the growth of compact colonies. The Aspergillus nidulans kinase gene is, thus, required for normal polarized growth at several stages of colony formation in the filamentous fungus A. nidulans.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Aspergillus nidulans / enzymology*
  • Aspergillus nidulans / genetics
  • Aspergillus nidulans / growth & development*
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases / chemistry
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics*
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
  • DNA, Fungal
  • Genes, Fungal
  • Molecular Sequence Data
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sequence Deletion

Substances

  • DNA, Fungal
  • Calcium-Calmodulin-Dependent Protein Kinases

Associated data

  • GENBANK/U59214