Regulation of IL-5 and IL-5 receptor expression in the bone marrow of allergic asthmatics

Int Arch Allergy Immunol. Feb-Apr 1999;118(2-4):101-3. doi: 10.1159/000024040.


Background: Following consistent demonstrations of the clinical relevance of fluctuations in eosinophil-basophil (Eo-B) progenitors in the blood of patients with a variety of allergic airway disorders, we have turned our attention recently to hemopoietic events occurring in the bone marrow of allergic asthmatic subjects, utilizing a model of airway allergen challenge.

Methods: Flow-cytometric analyses of CD34/45+ progenitors for coexpression of surface alpha-receptor subunits for IL-3, IL-5 and GM-CSF, as well as in situ hybridization and in situ PCR methodologies to detect mRNA for IL-5 and GM-CSF in developing Eo-B in colony and liquid culture assays were employed before and after in vivo allergen challenge.

Results: An early, specific upregulation of IL-5R alpha expression on CD34/45 progenitors was observed after allergen challenge, concomitant with the development of the late-phase asthmatic response. Protein and mRNA for both GM-CSF and IL-5 were expressed in a time-dependent manner ex vivo, in developing (beta 7-integrin-positive), colony-derived Eo-B after allergen challenge in vivo. Both retinoic acid and corticosteroids were able to downregulate IL-3- and IL-5-induced expression of IL-5R on cord-blood-derived as well as HL-60 cloned Eo-B progenitors.

Conclusion: These studies indicate the critical involvement of IL-5 and IL-5R in the induction of Eo-B differentiation and eosinophilic airway inflammation in allergic asthmatics, and point to these events as potential targets for long-term therapy of atopic disease.

MeSH terms

  • Asthma / immunology*
  • Asthma / pathology
  • Bone Marrow Cells / immunology*
  • Bone Marrow Cells / pathology
  • Cell Differentiation / immunology
  • Eosinophils / immunology
  • Eosinophils / pathology
  • Humans
  • Interleukin-5 / biosynthesis
  • Interleukin-5 / immunology*
  • Receptors, Interleukin / biosynthesis
  • Receptors, Interleukin / immunology*
  • Receptors, Interleukin-5


  • Interleukin-5
  • Receptors, Interleukin
  • Receptors, Interleukin-5