Charge derivatization of peptides for analysis by mass spectrometry

Mass Spectrom Rev. 1998 Jul-Aug;17(4):255-74. doi: 10.1002/(SICI)1098-2787(1998)17:4<255::AID-MAS1>3.0.CO;2-4.


The analysis of peptide derivatives by fast atom bombardment, liquid secondary-ionization mass spectrometry, plasma desorption, electrospray ionization, and matrix-assisted laser desorption/ionization is reviewed. The fragmentation patterns of peptides and of charge-derivatized peptides are compared, and the proposed fragment ion structures are summarized. A variety of derivatization approaches and the distinguishing features of mass spectra produced from these derivatives are described. The most promising derivatization approaches are evaluated, and the strengths and limitations of these approaches are discussed.

Publication types

  • Review

MeSH terms

  • Amino Acid Sequence
  • Indicators and Reagents
  • Mass Spectrometry / methods*
  • Organophosphorus Compounds
  • Peptides / chemistry*
  • Quaternary Ammonium Compounds
  • Spectrometry, Mass, Fast Atom Bombardment / methods
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Spectrometry, Mass, Secondary Ion / methods


  • Indicators and Reagents
  • Organophosphorus Compounds
  • Peptides
  • Quaternary Ammonium Compounds