Non-proteolytic activation of human prorenin by anti-prorenin prosegment (pf#1: 1P-15P) antiserum

Biosci Biotechnol Biochem. 1999 Mar;63(3):550-4. doi: 10.1271/bbb.63.550.


Recombinant human prorenin was activated by incubation with anti-prorenin prosegment (L1PPTDTTTFKRIFLKR15P) antiserum at 4 degrees C. This activation was dependent on the concentration of the antiserum and incubation time. After the activation no molecular weight alteration of prorenin was observed by immunoblotting analysis. A peptide of L1PPTDTTTF8P as well as L1PPTDTTTFKRIFLKR15P potently interfered with the activation. Most of the activated prorenin bound to Protein A Sepharose CL 4B. The Km and Vmax values of the activated prorenin were 0.2 microM and 23.7 micrograms Ang I/ml/h, respectively, which were similar in level to those of mature renin obtained by trypsinization.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Cold Temperature
  • Cricetinae
  • Enzyme Activation / immunology
  • Enzyme Precursors / chemistry
  • Enzyme Precursors / immunology
  • Enzyme Precursors / metabolism*
  • Humans
  • Immune Sera / metabolism*
  • Kinetics
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Peptide Fragments / immunology*
  • Rabbits
  • Renin / chemistry
  • Renin / immunology
  • Renin / metabolism*


  • Enzyme Precursors
  • Immune Sera
  • Peptide Fragments
  • Renin