Generation of enhancer trap lines in Arabidopsis and characterization of expression patterns in the inflorescence

Plant J. 1999 Mar;17(6):699-707. doi: 10.1046/j.1365-313x.1999.00409.x.

Abstract

Eleven thousand, three hundred and seventy enhancer/promoter trap lines in Arabidopsis were generated via T-DNA transformation utilizing the binary vector pD991 that contains a minimal promoter fused to the uidA reporter gene. Overall 31% of the lines generated exhibit a staining pattern in the inflorescence. Flanking DNA has been cloned from 15 lines exhibiting inflorescence staining patterns by either thermal asymmetric interlaced PCR (TAIL-PCR), inverse PCR (IPCR), or partial library construction. Seeds from these lines are available from the ABRC and NASC Arabidopsis stock centers and DNA pools are available from the ABRC.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Base Sequence
  • Caulimovirus / genetics
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • DNA, Plant / genetics
  • Enhancer Elements, Genetic
  • Gene Expression
  • Genes, Plant
  • Genes, Reporter
  • Genetic Vectors
  • Glucuronidase / genetics
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Transformation, Genetic

Substances

  • DNA Primers
  • DNA, Bacterial
  • DNA, Plant
  • T-DNA
  • Glucuronidase