Inhibition of stem cell factor- and nerve growth factor-induced morphological change by wortmannin in mast cells

Arch Pharm Res. 1999 Apr;22(2):108-12. doi: 10.1007/BF02976532.

Abstract

Recombinant murine stem cell factor (rmSCF) or recombinant murine nerve growth factor (rmNGF) induced the morphological change of large numbers of rat peritoneal mast cells (RPMC). We investigated the role of phosphatidylinositol 3'-kinase (PI3-kinase) in receptors-mediated morphological change in RPMC. Exposure of RPMC to PI3-kinase inhibitor, wortmannin, before the addition of rmSCF and rmNGF antagonized those factors-induced morphological change. These results suggest that the Pl3-kinase is involved in the signal transduction pathway responsible for morphological change following stimulation of rmSCF and rmNGF and that wortmannin blocks these responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androstadienes / pharmacology*
  • Animals
  • Cells, Cultured
  • Enzyme Inhibitors / pharmacology*
  • Male
  • Mast Cells / drug effects*
  • Mast Cells / pathology
  • Nerve Growth Factors / antagonists & inhibitors
  • Nerve Growth Factors / pharmacology*
  • Phosphatidylinositol 3-Kinases / physiology*
  • Phosphoinositide-3 Kinase Inhibitors
  • Rats
  • Rats, Wistar
  • Recombinant Proteins / pharmacology
  • Stem Cell Factor / antagonists & inhibitors
  • Stem Cell Factor / pharmacology*
  • Wortmannin

Substances

  • Androstadienes
  • Enzyme Inhibitors
  • Nerve Growth Factors
  • Phosphoinositide-3 Kinase Inhibitors
  • Recombinant Proteins
  • Stem Cell Factor
  • Wortmannin