Evidence that the trans-10,cis-12 isomer of conjugated linoleic acid induces body composition changes in mice

Lipids. 1999 Mar;34(3):235-41. doi: 10.1007/s11745-999-0358-8.


We investigated the effects of conjugated linoleic acid (CLA) preparations, which were enriched for the cis-9,trans-11 CLA isomer or the trans-10,cis-12 CLA isomer, on body composition in mice. Body composition changes (reduced body fat, enhanced body water, enhanced body protein, and enhanced body ash) were associated with feeding the trans-10,cis-12 CLA isomer. In cultured 3T3-L1 adipocytes, the trans-10,cis-12 isomer reduced lipoprotein lipase activity, intracellular triacylglycerol and glycerol, and enhanced glycerol release into the medium. By contrast, the cis-9,trans-11 and trans-9,trans-11 CLA isomers did not affect these biochemical activities. We conclude that CLA-associated body composition change results from feeding the trans-10,cis-12 isomer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells / drug effects
  • 3T3 Cells / metabolism
  • Adipocytes / drug effects
  • Adipocytes / metabolism
  • Animals
  • Body Composition / drug effects*
  • Body Weight / drug effects
  • Dose-Response Relationship, Drug
  • Female
  • Isomerism
  • Linoleic Acids / chemistry
  • Linoleic Acids / pharmacology*
  • Lipoprotein Lipase / drug effects
  • Lipoprotein Lipase / metabolism
  • Male
  • Mice
  • Mice, Inbred ICR
  • Triglycerides / metabolism


  • Linoleic Acids
  • Triglycerides
  • Lipoprotein Lipase