Isolation and mapping of a putative b subunit of human ATP synthase (ATP-BL) from human leukocytes

DNA Res. 1999 Feb 26;6(1):29-35. doi: 10.1093/dnares/6.1.29.


From a human-leukocyte cDNA library, we cloned cDNA encoding a novel protein, which has a significant homology with the b subunit of ATP synthase (proton-transporting ATPase, F1F0-ATPase; EC3.6.1.34) derived from Anabaena sp. strain PCC 7120. The cDNA has an open reading frame of 1314 nucleotides corresponding to 438 amino acids. The coding sequence was 37.9% identical over 57 amino acid with b subunit of ATP synthase. The 34-amino-acid region of the predicted peptide sequence displays a coiled-coil motif that could form a complex with some other protein(s). We designated this novel gene as ATP-BL because of its homology to the b subunit of ATP synthase. The ATP-BL locus was mapped by fluorescence in situ hybridization (FISH) and radiation hybrid mapping to the q24 region of chromosome 16.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 16
  • Cloning, Molecular
  • Humans
  • In Situ Hybridization, Fluorescence
  • Leukocytes / enzymology*
  • Molecular Sequence Data
  • Physical Chromosome Mapping
  • Proton-Translocating ATPases / genetics*
  • Proton-Translocating ATPases / physiology
  • Sequence Homology, Amino Acid
  • Tissue Distribution


  • Proton-Translocating ATPases

Associated data

  • GENBANK/AB018551