Regulation of blood pressure during long-term ouabain infusion in Long-Evans rats

Am J Hypertens. 1999 Apr;12(4 Pt 1):423-6. doi: 10.1016/s0895-7061(98)00250-7.

Abstract

We tested whether ouabain, an inhibitor of the sodium pump, can lead to chronic hypertension in Long-Evans rats using sensitive 24-h measurements of blood pressure. After a control week of vehicle isotonic saline infusion (14.4 mL/day), ouabain was infused intravenously at 30 microg/kg/day in intact (2K) and uninephrectomized (1K) Long-Evans rats for a total of 4 weeks. Although plasma ouabain concentration rose to 0.97 +/- 0.15 nmol/L with ouabain infusion, mean arterial pressure did not change in either 2K (delta = -0.6 +/- 1.3 mm Hg) or 1K (delta = -1.2 +/- 0.7 mm Hg) rats. These data suggest that Long-Evans rats are insensitive to the hypertensive effects of ouabain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Pressure / drug effects*
  • Enzyme Inhibitors / pharmacology*
  • Glomerular Filtration Rate / drug effects
  • Heart Rate / drug effects
  • Infusions, Intravenous
  • Male
  • Ouabain / blood
  • Ouabain / pharmacology*
  • Potassium / blood
  • Potassium / urine
  • Rats
  • Rats, Long-Evans
  • Sodium / blood
  • Sodium / urine
  • Time Factors

Substances

  • Enzyme Inhibitors
  • Ouabain
  • Sodium
  • Potassium