Abstract
A cell-free system of protein synthesis from rat heart muscle is described. The system contained preparations of ribosomes, which were not separated from myofibrillar proteins. The conditions for incorporation of 14C-leucine into the polyribosomes of the fraction were established and its protein synthetizing activity was studied.
MeSH terms
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Animals
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Carbon Radioisotopes
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Cell-Free System*
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In Vitro Techniques
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Isotope Labeling
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Male
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Myocardium / cytology*
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Phosphatidylcholines
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RNA, Ribosomal / analysis
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Rats
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Ribosomal Proteins / analysis
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Ribosomal Proteins / biosynthesis*
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Ribosomes / metabolism*
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Spectrophotometry
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Subcellular Fractions*
Substances
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Carbon Radioisotopes
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Phosphatidylcholines
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RNA, Ribosomal
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Ribosomal Proteins