In this study of the medial geniculate body in the tree shrew eight subdivisions are identified on the basis of differences recognized in Nissl-stained material. Experiments using the methods of anterograde and retrograde axonal transport and anterograde degeneration show that each subdivision has a unique pattern of connections with the midbrain. The ventral division of the medial geniculate body contains at least two subdivisions, the ventral nucleus and the caudomarginal nucleus. The ventral nucleus is characterized by densely-packed cells and receives topographically organized projections from the central nucleus of the inferior colliculus. The caudomarginal nucleus, on the other hand, receives its major midbrain projections from the medial nucleus in the inferior colliculus. In the dorsal division four subdivisions are distinguished. The suprageniculate nucleus contains large, loosely-packed cells and receives projections from the deep layers of the superior colliculus and from the midbrain tegmentum. The dorsal nucleus receives projections from the midbrain tegmentum. The deep dorsal and anterodorsal nuclei have neurons which resemble those in the dorsal nucleus. Both receive projections from the roof nucleus of the inferior colliculus but the deep dorsal nucleus receives an additional projection from the parabrachial tegmentum. The medial division has a rostral and a caudal subdivision. The ascending projections to the rostral nucleus are from the lateral zone in the inferior colliculus and from the spinal cord. The caudal nucleus contains cells with large somas and receives projections from most of the midbrain areas which project to the other subdivisions of the medial geniculate body.