The selection of Chinese hamster cells deficient in oxidative energy metabolism

Somatic Cell Genet. 1976 Jul;2(4):331-44. doi: 10.1007/BF01538838.


A selection scheme based on the nutritional requirements of a previously described respiration-deficient Chinese hamster line has been used to isolate new mutants defective in oxidative energy metabolism. Three of the primary characteristics of this type of mutant are (1) a strict dependency on the continued presence of glucose for survival; (2) a drastic reduction in the rate of oxygen consumption; (3) an inhibition of Krebs cycle activity resulting in auxotrophy for asparagine and carbon dioxide. In the case of one cell line which was used (V79), up to 65% of the survivors of a selection were found to possess this phenotype after only one round of selection. By contrast, it proved much more difficult to obtain such mutants from another cell line (CCL16). A preliminary characterization of a number of these mutants is presented.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Asparagine / metabolism
  • Carbon Dioxide / metabolism
  • Cell Line
  • Cell Survival
  • Cells, Cultured
  • Citric Acid Cycle
  • Cricetinae
  • Energy Metabolism*
  • Glucose / metabolism
  • In Vitro Techniques
  • Mutation*
  • Oxygen Consumption
  • Phenotype
  • Selection, Genetic


  • Carbon Dioxide
  • Asparagine
  • Glucose